Urban land uses, including educational institutions, especially universities, have a significant share in the consumption of natural resources and the production of pollutants, and consequently exert considerable impacts on the environment. Due to the service-oriented nature and the wide range of educational, research, and support activities, universities consume large amounts of energy, water, and other resources. Therefore, accurate and comprehensive assessment of their environmental impacts through indicators such as the ecological footprint is an undeniable necessity for sustainable resource management and reducing environmental consequences. The present study was conducted with the aim of evaluating the ecological footprint of Gorgan University of Agricultural Sciences and Natural Resources in the new campus area, using the life cycle assessment approach. In this study, by identifying the consumed resources and related environmental flows, an attempt was made to provide a quantitative picture of the impacts resulting from university activities. The results of this research can be used as a basis for optimizing consumption patterns, developing environmental strategies, and moving towards green universities. Considering the significant share of universities in resource consumption and pollutant production, and the lack of comprehensive and scientific assessments of the environmental impacts of educational institutions in the country, conducting this research with a life cycle approach is an important step towards improving sustainable management and enhancing the environmental performance of universities. In this study, in addition to the ecological footprint by the EF method, the water footprint by the AWARE method, and also the carbon footprint by the IPCC method in 2023 were examined. The total number of staff and students of Gorgan University of Agricultural Sciences and Natural Resources in the campus unit is 4,121. The final estimated ecological footprint score of Gorgan University of Agricultural Sciences and Natural Resources is -2.75 pt. The greatest negative impact on the university’s ecological footprint relates to electricity consumption, and in the impact categories, to climate change, followed by resource consumption, while the greatest environmentally compatible effect on the university’s ecological footprint is related to water storage and the university’s water recycling system. The results obtained from examining the carbon footprint show that the final value obtained for the carbon footprint of Gorgan University of Agricultural Sciences and Natural Resources is -2.17 × 104 kilograms of carbon dioxide equivalent. Water storage is one of the most effective solutions in reducing the carbon footprint, since this action, by reducing dependence on pumping operations, significantly reduces energy consumption and ultimately leads to a reduction in greenhouse gas emissions. Electricity consumption at the university has increased its carbon footprint. The total estimated score for the water footprint of Gorgan University of Agricultural Sciences and Natural Resources is -1.06 × 104. The measures presented, such as creating water storage and recycling systems and green spaces at the university, have positive and environmentally compatible effects. Analysis of the ecological footprint of universities using the life cycle approach can be used as a model for other educational institutions to reduce environmental impacts and for effective planning. This study shows that accurate and comprehensive assessments are an effective step in optimizing resource consumption and protecting the environment. Overall, this research, through a multidimensional evaluation of environmental impacts, reveals the importance of resource management and the implementation of sustainable solutions in universities and can pave the way for improving the environmental performance of educational institutions.