نوع مقاله : مقاله علمی - پژوهشی

نویسندگان

1 گروه زیست شناسی دریا، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران

2 گروه شیمی، دانشکده علوم پایه، دانشگاه هرمزگان، بندرعباس، ایران

3 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه قم، قم، ایران

4 پژوهشکده اکولوژی خلیج فارس و دریای عمان، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و تزویج کشاورزی، بندرعباس، ایران

چکیده

تقاضای روزافزون برای استفاده از فناوری نانو در سال‌های اخیر منجر به انتشار نانومواد در محیط‌زیست شده است. هدف از مطالعه حاضر، سنتز نانوذرات مبتنی بر اکسیدگرافن با استفاده از عصاره آبی جلبک دریایی سبز و ارزیابی سمیت آن‌ها در مقابل ریزجلبک Chaetoceros muelleri به عنوان یک خوراک زنده مهم در پرورش آبزیان است. در مطالعه آزمایشگاهی حاضر، ابتدا فرایند سنتز زیستی نانوذرات گرافن با استفاده از عصاره آبی جلبک سبز Ulva flexuosa انجام شد و در ادامه ارزیابی فعالیت ضدجلبکی و تعیین میزان رنگدانه‌های فتوسنتزی و پروتئین ریزجلبک صورت پذیرفت. نتایج مربوط به آنالیزهای مشخصه‌یابی، احیا اکسیدگرافن به کمک عصاره آبی جلبک سبز U. flexuosa و تبدیل آن به گرافن را تأیید کرد. ارزیابی فعالیت ضدجلبکی نمونه‌های مورد مطالعه نشان داد که اکسیدگرافن احیا شده دارای فعالیت ضدجلبکی بالاتری نسبت به اکسیدگرافن است. هم­چنین نتایج نشان داد که با افزایش غلظت نانوذرات سنتز شده میزان رنگدانه‌های فتوسنتزی و پروتئین کاهش یافت. اثرات سمی نانوذرات آزاد شده می‌تواند به ذخایر C. muelleri به عنوان یک خوراک زنده مهم در پرورش آبزیان آسیب برساند.

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